RESULTS

New Paper: Extracellular Vscs Analysis in the COVID19 Era: Insights on Serum Inactivation Protocols towards downstream Isolation and Analysis

Frigerio R , Musicò A, Brucale M, Ridolfi  A, Galbiati  S, Vago R, Bergamaschi G, Ferretti  AM, Chiari M, Valle  F, Gori A, Cretich M.

Cells. 2021 Mar 4;10(3):544.

DOI: https://doi.org/10.3390/cells10030544

Repositorylink: https://ec.europa.eu/info/funding-tenders/opportunities

New Paper: A plasmon-based nano-ruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vscs

Caselli L , Ridolfi A, Cardellini J, Sharpnack L, Paolini L, Brucale M, Valle F, Montis C, Bergese P, Berti D.

Nanoscale Horiz. 2021 Jun 28;6(7):543-550.

DOI: https://doi.org/10.1039/D1NH00012H

Repository link: https://chemrxiv.org/engage/chemrxiv/article-details/60c75363469df4e320f44d6d

Training Activities

Training Activities

D3.5 Integrated modular system for production of EVs

Integrated modular system forproduction of EVs

Paper: Nanoparticles and organized lipid assemblies: from interaction to design of hybrid soft devices.

Nanoparticles and organized lipid assemblies: from interaction to design of hybrid soft devices.
M. Mendozza, L. Caselli, A. Salvatore, C. Montis*and D. Berti*. Soft Matter, 2019,15, 8951-8970.

doi: https://doi.org/10.1039/C9SM01601E

Repository link: https://flore.unifi.it/handle/2158/1175074.2#.YDYmTpNKhsM

Paper: Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces

Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces
C. Montis, A. Salvatore, F. Valle, L. Paolini. F. Carlà, P. Bergese, D. Berti. J Colloid Interface Sci.  June 2020, 570: 340-349.

https://doi.org/10.1016/j.jcis.2020.03.014

https://flore.unifi.it/handle/2158/1218287#.YDYl05NKhsM

paper: evFOUNDRY researchers published a paper in Micromachines.

By combining DLD with electric fields (eDLD), Prof. Tegenfeldt's group show separation of a variety of nano and micro-sized particles primarily by their zeta potential – which is correlated to their surface charge.

https://www.mdpi.com/2072-666X/11/11/1014

Paper: AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles.

The evFOUNDRY consortium develops an AFM-based method for the high-throughput nanomechanical and morphological measurement of individual EVs

https://iris.unibs.it/handle/11379/534530

https://www.biorxiv.org/content/10.1101/854539v1

https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b05716

Paper: Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin.

https://doi.org/10.1080/20013078.2020.1741174

https://iris.unibs.it/handle/11379/529275

Paper: Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation

https://doi.org/10.1111/jmi.12910

https://doi.org/10.1101/2020.05.03.075101

Paper: Fluorescent Labeling of helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach

https://doi.org/10.3390/biomedicines8070213

https://pure.au.dk

Paper: Shedding light on membrane-templates clustering of gold nanoparticles

https://doi.org/10.1016/j.jcis.2020.03.123

https://iris.unibs.it/handle/11379/529276

Paper: CONAN for evFOUNDRY

evFOUNDRY researchers in collaboration with Andrea Zendrini - to date post-doc researcher at Università degli Studi di Brescia and at Università Cattolica di Piacenza - published a protocol that describes how to assign purity grade and titrate extracellular vesicle solutions of a few microliters. The assay is an augmented version of the COlorimetric NANoplasmonic (CONAN) assay developed in 2015 by Paolo Bergese's group. It has been implemented in the first year of activity of evFOUNDRY, being now part of the standard operating procedures (SOPs) for  preparing quality standard nanosized EVs from bovine milk and Ascaris media.

https://doi.org/10.3389/fbioe.2019.00452

https://iris.unibs.it/handle/11379/528221

EU Portal-Event Report: First clustering event on extracellular vesicles

https://ec.europa.eu/digital-single-market/en/news/first-clustering-event-extracellular-vesicles

Paper: Extracellular vesicles and nano-friends featured in Biomaterials Science back cover

evFOUNDRY researchers of the University of Brescia and CSGI explain on the first 2020 issue of Biomaterials Science how scientists are learning to exploit the wealth of nanoparticles that populate the secretome, such as extracellular vesicles and lipoproteins. The review article has been also featured in the issue back cover.

http://dx.doi.org/10.1039/c9bm01007f

https://iris.unibs.it/handle/11379/526980

Paper: Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop

https://www.tandfonline.com/doi/full/10.1080/20013078.2019.1684862

https://iris.unibs.it/handle/11379/526637

evMANIFESTO released.

The 1ST CLUSTERING EVENT FOR EXTRACELLULAR VESICLES FET-OPEN PROJECTS, held in Palermo in November 06th 2019, involved the 5 EU funded ongoing FET OPEN projects on EVs, counting participation of 34 research and industrial organizations from 15 EU countries. The first collective action of the newborn cluster was producing the attached position document, the evMANIFESTO, which aims at highlighting the current EU effort in EV research within the FET program and the urgency of introducing and securing more firmly the EV-related theme as a cornerstone in the research agenda in EU. 

Paper: Active Posts in Deterministic Lateral Displacement Devices (Open access)

 DOI link: https://doi.org/10.1002/admt.201900339

Repository link: http://zenodo.org/record/3352780#.XZHEh1Uzapo

Promo material. evFOUNDRY poster

Poster used to present evFOUNDRY at ISEV2019.

https://www.isev.org/page/ISEV2019

Exploitation activities

Dissemination activities

Communication activities

Paper: First study supported by evFOUNDRY published on Cells

http://doi.org/10.3390/cells7120273

http://hdl.handle.net/11379/515026

Paper: Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk on Journal of Extracellular Vesicles

http://doi.org/10.1080/20013078.2019.1578116

Paper: Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines” published online in the Journal of Extracellular Vesicles

http://doi.org/10.1080/20013078.2018.1535750

http://hdl.handle.net/11379/512523

Deliverable D5.1: Project website online and logo

Deliverable D6.1: Internal and main external communication channels